PURIFICATION AND CHARACTERIZATION OF A NOVEL PHOSPHOLIPID TRANSFER PROTEIN FROM FILAMENTOUS FUNGI

被引：12

作者：

GRONDIN, P

VERGNOLLE, C

CHAVANT, L

KADER, JC

机构：

[1] UNIV PARIS 06,PHYSIOL CELLULAIRE LAB,CNRS,UA 1180,4 PL JUSSIEU,F-75230 PARIS 05,FRANCE

[2] UNIV TOULOUSE 3,CRYPTOGAMIE LAB,F-31062 TOULOUSE,FRANCE

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1990年 / 22卷 / 01期

关键词：

D O I：

10.1016/0020-711X(90)90083-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. We have isolated from mycelia of Mucor mucedo, a filamentous fungus, a phospholipid transfer protein. 2. 2. The purification steps wer gel filtration, hydroxyapatite chromatography, blue affinity column and fast protein liquid chromatography on anion exchanger. 3. 3. A purified protein was obtained with a molecular mass of 24 kDa and a pI of 5.05 and its N-terminal sequence was established. 4. 4. This protein transfers phosphatidylinositol, as well as phosphatidylcholine and phosphatidyl-ethanolamine. © 1990.

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页码：93 / &

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