PINOCYTOSIS IN L-CELLS - ITS DEPENDENCE ON MEMBRANE STEROL AND THE CYTOSKELETON

被引:21
作者
HEINIGER, HJ
MARSHALL, JD
机构
[1] The Jackson Laboratory, Bar Harbor
关键词
D O I
10.1016/0309-1651(79)90002-X
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Pinocytosis in L-cells, grown in serum-free medium, was depressed when cultures were treated with oxygenated derivatives of cholesterol which inhibited sterol synthesis and reduced the sterol concentration of the plasma membranes. Noninhibitory sterols, such as cholesterol or desmosterol counteracted the effects of the inhibitors. Treatment with polylysine increased the rate of pinocytosis in sterol-depleted cells to a level similar to the enhanced rate found in polylysine treated control cells. Drugs which interfere with cytoskeletal systems (microfilaments, microtubules) also depressed pinocytosis but their effect could not be overcome by treatment with polylysine. The results suggest that pinocytosis requires both a critical fluidity in the plasma membrane and a functional cytoskeleton which interacts with the plasma membrane during the formation of endocytic vesicles. © 1979.
引用
收藏
页码:409 / 420
页数:12
相关论文
共 33 条
[1]  
ALLISON AC, 1971, NATURE-NEW BIOL, V232, P153
[2]   INFLUENCE OF BASIC POLYPEPTIDES ON PHASE-TRANSITION OF PHOSPHOLIPID LIPOSOMES [J].
BACH, D ;
MILLER, IR .
BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 433 (01) :13-19
[3]   AMEBOID MOVEMENT INDUCED IN CULTURED MACROPHAGES BY COLCHICINE OR VINBLASTINE [J].
BHISEY, AN ;
FREED, JJ .
EXPERIMENTAL CELL RESEARCH, 1971, 64 (02) :419-+
[4]   ALTERED MOVEMENT OF ENDOSOMES IN COLCHICINE-TREATED CULTURED MACROPHAGES [J].
BHISEY, AN ;
FREED, JJ .
EXPERIMENTAL CELL RESEARCH, 1971, 64 (02) :430-&
[5]   EFFECT OF PARTIAL REPLACEMENTS OF MEMBRANE CHOLESTEROL BY OTHER STEROIDS ON OSMOTIC FRAGILITY AND GLYCEROL PERMEABILITY OF ERYTHROCYTES [J].
BRUCKDORFER, KR ;
DEMEL, RA ;
DEGIER, J ;
VANDEENE.LL .
BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 183 (02) :334-+
[6]  
Chen H W, 1978, Prog Exp Tumor Res, V22, P275
[7]   RELATIONSHIP BETWEEN STEROL SYNTHESIS AND DNA-SYNTHESIS IN PHYTOHEMAGGLUTININ-STIMULATED MOUSE LYMPHOCYTES [J].
CHEN, HW ;
HEINIGER, HJ ;
KANDUTSCH, AA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (05) :1950-1954
[8]   INHIBITION OF CELL-GROWTH BY OXYGENATED DERIVATIVES OF CHOLESTEROL [J].
CHEN, HW ;
KANDUTSCH, AA ;
WAYMOUTH, C .
NATURE, 1974, 251 (5474) :419-421
[9]  
CHEN HW, 1978, J BIOL CHEM, V253, P3180
[10]   MODIFICATION OF RED-CELL MEMBRANE STRUCTURE BY CHOLESTEROL-RICH LIPID DISPERSIONS - MODEL FOR PRIMARY SPUR CELL DEFECT [J].
COOPER, RA ;
ARNER, EC ;
WILEY, JS ;
SHATTIL, SJ .
JOURNAL OF CLINICAL INVESTIGATION, 1975, 55 (01) :115-126