REMOVAL OF DNA FROM RNA BY CHROMATOGRAPHY ON ACETYLATED N-[N'-(META-DIHYDROXYLBORYLPHENYL)SUCCINAMYL]AMINOETHYL CELLULOSE

被引：13

作者：

ACKERMAN, S

COOL, B

FURTH, JJ

机构：

[1] Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 100卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90128-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA synthesized in vitro can be separated from contaminating DNA by chromatography on acetylated N-[N′-(m-dihydroxyborylphenyl)succinamyl]aminoethyl cellulose. The recovery of RNA is greater than 95%, and essentially all the DNA is removed. This procedure is extremely useful in studies in which DNA contamination of RNA interferes with subsequent analysis. © 1979.

引用

页码：174 / 178

页数：5

共 9 条

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[2] ENZYMIC POLYADENYLATION OF 5S-RIBOSOMAL RIBONUCLEIC-ACID AND SYNTHESIS OF A COMPLEMENTARY DEOXYRIBONUCLEIC-ACID
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[3] RNA SYNTHESIZED INVITRO BY CALF THYMUS RNA POLYMERASE-III (C), AS WELL AS BY ESCHERICHIA-COLI RNA-POLYMERASE, IS RESTRICTED TO A SUBSET OF CALF THYMUS DNA
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[5] ISOLATION OF TRANSFER-RNA ISOACCEPTORS BY CHROMATOGRAPHY ON DIHYDROXYBORYL-SUBSTITUTED CELLULOSE, POLYACRYLAMIDE, AND GLASS
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[6] TRANSCRIPTION OF CHROMATIN BY AN RNA-POLYMERASE OF CALF THYMUS WHICH IS SENSITIVE TO HIGH-CONCENTRATIONS OF ALPHA-AMANITIN
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[7] IMPROVED METHOD FOR PURIFICATION OF TRANSFER-RNA BY CHROMATOGRAPHY ON DIHYDROXYBORYL SUBSTITUTED CELLULOSE
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[J]. NUCLEIC ACIDS RESEARCH, 1975, 2 (06) : 853 - 864
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[J]. NUCLEIC ACIDS RESEARCH, 1974, 1 (05) : 653 - 671
[9] SYNTHESIS OF CELLULOSE DERIVATIVES CONTAINING DIHYDROXYBORYL GROUP AND A STUDY OF THEIR CAPACITY TO FORM SPECIFIC COMPLEXES WITH SUGARS AND NUCLEIC ACID COMPONENTS
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