EFFECT OF EXOGENOUS DNA FRAGMENTS ON HUMAN CELL EXTRACT-MEDIATED DNA-REPAIR SYNTHESIS

被引：51

作者：

BIGGERSTAFF, M ^{[1
]}

ROBINS, P ^{[1
]}

COVERLEY, D ^{[1
]}

WOOD, RD ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND, CLARE HALL LABS, S MIMMS EN6 3LD, HERTS, ENGLAND

来源：

MUTATION RESEARCH | 1991年 / 254卷 / 03期

关键词：

ULTRAVIOLET LIGHT; UV; DNA REPAIR; REPAIR SYNTHESIS; HUMAN CELL EXTRACTS;

D O I：

10.1016/0921-8777(91)90059-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Extracts from HeLa cells were used to study the susceptibility of repair synthesis in UV-irradiated plasmid DNA to inhibition by exogenously added nucleic acid. Purified DNA restriction fragments have little inhibitory effect on repair synthesis. However, activated calf thymus DNA fragments, genomic DNA fragments in cell extracts, and sonicated plasmid DNA all inhibited repair synthesis. Degraded DNA fragments arising from E. coli during bacterial plasmid purification were found to be particularly inhibitory. tRNA is not a potent inhibitor of in vitro repair synthesis. In order to observe efficient DNA repair synthesis mediated by human cell extracts, it is essential to prepare highly purified closed circular plasmid DNA, and we describe a reliable method for doing so.

引用

页码：217 / 224

页数：8

共 16 条

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