STRUCTURE AND EXPRESSION OF HUMAN AND RAT D2-DOPAMINE RECEPTOR GENES

被引：20

作者：

ARAKI, K

KUWANO, R

MORII, K

HAYASHI, S

MINOSHIMA, S

SHIMIZU, N

KATAGIRI, T

USUI, H

KUMANISHI, T

TAKAHASHI, Y

机构：

[1] NIIGATA UNIV,BRAIN RES INST,DEPT NEUROPHARMACOL,MOLEC BIOL RES LAB,NIIGATA 951,JAPAN

[2] NIIGATA UNIV,BRAIN RES INST,DEPT NEUROPATHOL,NIIGATA 951,JAPAN

[3] SAIGATA NATL HOSP,OHGATA,NIIGATA 94931,JAPAN

[4] KEIO UNIV,SCH MED,DEPT MOLEC BIOL,SHINJUKU KU,TOKYO 160,JAPAN

来源：

NEUROCHEMISTRY INTERNATIONAL | 1992年 / 21卷 / 01期

关键词：

D O I：

10.1016/0197-0186(92)90071-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

D2 dopamine receptor may be related with the pathogenesis of Parkinson's disease and schizophrenia. Furthermore, the antipsychotic drugs have high affinity for D2 dopamine receptor. We carried out the cloning of the genomic DNA for human D2 dopamine receptor and clarified the structure of this gene. Our isolated gene spans about 15 kbp and consists of seven exons interrupted by six introns. However, putative first exon was not yet identified. Spot blot hybridization analysis of cell sorter fractionated human chromosomal DNA with D2 receptor genomic DNA revealed the localization of this gene in the chromosome 11 fraction. We analyzed human genomic DNA by Southern blot hybridization with D2 dopamine receptor genomic DNA as a probe, but so far we could not find RFLP. Northern blot analyses of brain RNA of several animals and rat brain RNA after various treatments were carried out. Developmental changes of D2 dopamine receptor mRNA were observed in the rat brains.

引用

页码：91 / 98

页数：8

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