6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE FROM SALMON LIVER - AMINO-ACID-SEQUENCE ANALYSIS BY TANDEM MASS-SPECTROMETRY

被引：16

作者：

HAUER, CR

LEIMBACHER, W

HUNZIKER, P

NEUHEISER, F

BLAU, N

HEIZMANN, CW

机构：

[1] UNIV ZURICH,DEPT PEDIAT,DIV CLIN CHEM,STEINWIESSTR 75,CH-8032 ZURICH,SWITZERLAND

[2] UNIV ZURICH IRCHEL,INST BIOCHEM,CH-8052 ZURICH,SWITZERLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91824-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The most frequent variant of atypical phenylketonuria, an inborn error of metabolism, is characterized by a low activity of the 6-pyruvoyl tetrahydropterin synthase. We purified and characterized this enzyme from salmon liver known to contain high levels. After digestion, peptides were sequenced by tandem mass spectrometry and/or automated Edman microsequence analysis. Both a free amine terminus and an N-acetylated amine terminus were found, indicating the presence of two isoforms. The peptide sequences determined here have a high degree of homology with the protein sequence deduced from cDNA for rat 6-pyruvoyl tetrahydropterin synthase (1), however, the amine termini of these proteins differ significantly. © 1992.

引用

页码：953 / 959

页数：7

共 13 条

[1]

BLAU N, 1988, ANNU REV NUTR, V8, P185

[2] BIOSYNTHESIS AND FUNCTION OF TETRAHYDROBIOPTERIN [J].