STOICHIOMETRY OF THE BINDING OF CHROMOSOMAL PROTEIN MC1 FROM THE ARCHAEBACTERIUM, METHANOSARCINA SPP CHT155, TO DNA

被引：16

作者：

CULARD, F ^{[1
]}

LAINE, B ^{[1
]}

SAUTIERE, P ^{[1
]}

MAURIZOT, JC ^{[1
]}

机构：

[1] INST PASTEUR,F-59019 LILLE,FRANCE

来源：

FEBS LETTERS | 1993年 / 315卷 / 03期

关键词：

CHROMOSOMAL PROTEIN; BINDING SITE; MC1; HU PROTEIN;

D O I：

10.1016/0014-5793(93)81189-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the binding stoichiometry of the chromosomal MC1 protein on DNA using the gel retardation technique. Analysis of the distribution of the complex containing 0, 1, 2, 3...... bound proteins shows that the protein MC1 interacts with the DNA as a monomer. Binding experiments with short DNA fragments of various lengths shows that the site size is 11 bp in length. These results are compared to those obtained with other chromosomal proteins including HU protein.

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页码：335 / 339

页数：5

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