EFFECT OF PARATHYROID EXTRACT ON ALKALINE PHOSPHATASE ACTIVITY OF SURFACE OF ISOLATED BONE CELLS

被引：25

作者：

HEKKELMA.JW

MOSKALEW.S

机构：

[1] Laboratory for Cell Biology and Histology, University of Leiden, Leiden

[2] Department of Histology and Embryology, School of Medicine, Warsaw

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 58卷 / 2-3期

关键词：

D O I：

10.1016/0014-4827(69)90506-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A method is described to measure the alkaline phosphatase activity of the surface of isolated bone cells. The enzyme activity was determined by incubating the cells in a medium of pH 8.5 with NADP as the substrate. The amount of NAD formed by the phosphatase reaction was determined spectrofotometrically after reduction with alcohol dehydrogenase and ethanol. Parathyroid extract (1 IU/ml) caused a decrease of approx. 40% in the surface-bound phosphatase activity within 2 h. © 1969.

引用

页码：283 / &

共 10 条

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