ACTION OF DETERGENTS ON COVALENTLY LABELED, MEMBRANE-BOUND MUSCARINIC ACETYLCHOLINE-RECEPTOR OF BOVINE NUCLEUS CAUDATUS

被引：25

作者：

RUESS, KP

LIEFLANDER, M

机构：

[1] Institut für Chemie der Universität Regensburg, D-8400 Regensburg

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)92094-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The muscarinic acetylcholinereceptor associated with highly purified neuronal membranes from bovine nucleus caudatus was covalently labelled with N-[2′,3′-3H2]propyl-N-2-aziridiniumethylbenzilate. The influence of various detergents on binding activity of the membrane bound receptor and on solubilization of the labelled receptor was studied. A constituent part of the muscarinic acetylcholinereceptor, which is easily extracted by certain detergents without extracting the labelled protein, seems to be partially responsible for binding activity of the receptor protein. Sodiumdodecylsulfate-gradient-gelelectrophoresis reveals that the labelled subunit has a molecular weight of 75 000. © 1979.

引用

页码：627 / 633

页数：7

共 8 条

[1]

ARONSTAM RS, 1978, LIFE SCI, V23, P1377

[2] ROLE OF PHOSPHOLIPIDS IN MUSCARINIC BINDING BY NEURAL MEMBRANES [J].