DETECTION OF IGG ANTIBODIES SPECIFIC FOR MEASLES-VIRUS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

被引：26

作者：

KAHANE, S ^{[1
]}

GOLDSTEIN, V ^{[1
]}

SAROV, I ^{[1
]}

机构：

[1] BEN GURION UNIV NEGEV,SOROKA MED CTR,BEER SHEVA 84120,ISRAEL

来源：

INTERVIROLOGY | 1979年 / 12卷 / 01期

关键词：

Complement fixation; Hemagglutination inhibition; Measles ELISA;

D O I：

10.1159/000149067

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A solid-phase, enzyme-linked immunosorbent assay (ELISA) for determination of IgG antibodies against measles virus is described. The assay utilized antigen-coated polystyrene microplates. The antigen consisted of a sonicated extract of measles-infected Vero cells. Goat anti-human IgG-peroxidase conjugate was used to detect human IgG bound to viral antigen. Sera taken from 63 healthy adults, 11 young children and 36 patients were evaluated for their IgG titer against measles virus. Comparison of results obtained by ELISA with those obtained by hemagglutination-inhibition (HI) assay or by complement fixation showed good agreement between the tests. The geometric mean titer (GMT) for healthy adults was 753 for ELISA and 32.8 for HI. If these averages are taken as a measure of comparison, then ELISA is approximately 23 times more sensitive than HI. ELISA technique is rapid to perform and could be recommended for routine diagnosis. © 1979 S. Karger AG, Basel.

引用

页码：39 / 46

页数：8

共 20 条

[1] SOLID-PHASE RADIOIMMUNOASSAY FOR IGG AND IGM ANTIBODIES AGAINST MEASLES-VIRUS [J].