FRUCTOSE 1,6-DIPHOSPHATASE FROM RABBIT LIVER .13. NUMBER OF MN++ BINDING SITES MEASURED WITH 54MN++

被引：21

作者：

PONTREMOLI, S

GRAZI, E

ACCORSI, A

机构：

[1] Istituto di Chimica Biologics dell'Universita di Ferrara, Ferrara

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 37卷 / 04期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(69)90851-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The requirement of rabbit liver FDPase for a divalent cation for catalytic activity has now been correlated with the binding of 54Mn++ in gel filtration studies. The enzyme binds four equivalents of 54Mn++ at neutral pH and four additional equivalents above pH 8.5. Only the binding of the first set of four equivalents is required for catalytic activity, and the pK of binding is approximately 7. The results provide additional support for the 4-subunit model for the enzyme. © 1969.

引用

页码：597 / +

页数：1

共 8 条

[1] A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER [J].