EFFECT OF ENZYME-ACTIVATED INHIBITORS ON ORNITHINE DECARBOXYLASE AND GROWTH OF BOTRYTIS-CINEREA

被引：12

作者：

SMITH, TA ^{[1
]}

BARKER, JHA ^{[1
]}

JUNG, M ^{[1
]}

机构：

[1] DOW ELANCO LTD,WANTAGE,OXON,ENGLAND

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 06期

关键词：

Botrytis cinerea; Deuteromycotina; difluoromethylornithine; enzyme-activated inhibition; fungi; ornithine decarboxylase; polyamines; putrescine;

D O I：

10.1016/0031-9422(90)85010-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme ornithine decarboxylase (ODC) from Botrytis cinerea has been purified 68-fold with 74% recovery. The optimum pH was 7.3 and the Km for ornithine was 70μM. Using this preparation, the effects of several potential enzyme-activated inhibitors of ODC have been studied, comparing their properties with those of difluoromethylornithine (DFMO) which is a known growth inhibitor for Botrytis and other fungi. Difluoromethylarginine and dehydromonofluoromethylarginine, which are at least as effective as DFMO for the inhibition of growth of Botrytis cinerea, are relatively poor inhibitors of ODC. R,R-Methylacetylenicputrescine and dehydromonofluoromethylornithine, which are relatively poor inhibitors of Botrytis growth, are very efficient inhibitors of the ODC. © 1982.

引用

页码：1759 / 1762

页数：4

共 31 条

[1] FORMATION OF N-CARBAMYL PUTRESCINE FROM CITRULLINE IN ESCHERICHIA-COLI [J].