MEASUREMENT OF 2-DEOXYGLUCOSE AND 2-DEOXYGLUCOSE 6-PHOSPHATE IN TISSUES

被引：31

作者：

MANCHESTER, JK

CHI, MMY

CARTER, JG

PUSATERI, ME

MCDOUGAL, DB

LOWRY, OH

机构：

[1] Department of Pharmacology, Washington University School of Medicine, St. Louis

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90265-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymatic methods previously described for 2-deoxyglucose (DG) and 2-deoxyglucose 6-phosphate have been refined and adapted to measurements of brain samples ranging from 50 mg wet weight to less than a microgram dry weight. Procedures for preparing such samples for assay are described. Analytical properties of the enzymes employed are given together with means for overcoming their possible short comings. Emphasis is placed on information useful for employing DG to assess rapid changes in glucose metabolism. © 1990.

引用

页码：118 / 124

页数：7

共 6 条

[1] ENZYMATIC ASSAYS FOR 2-DEOXYGLUCOSE AND 2-DEOXYGLUCOSE 6-PHOSPHATE
CHI, MMY
PUSATERI, ME
CARTER, JG
NORRIS, BJ
MCDOUGAL, DB
LOWRY, OH
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 161 (02) : 508 - 513
[2] IMPROVED ENZYMATIC CYCLE FOR NICOTINAMIDE-ADENINE DINUCLEOTIDE PHOSPHATE
CHI, MMY
LOWRY, CV
LOWRY, OH
[J]. ANALYTICAL BIOCHEMISTRY, 1978, 89 (01) : 119 - 129
[3] CORRECTING A POTENTIAL DEFECT IN AN ENZYMATIC CYCLE FOR NADP
HINTZ, CS
CHI, MMY
LOWRY, OH
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 128 (01) : 186 - 190
[4] Lowry O.H., 1972, FLEXIBLE SYSTEM ENZY
[5] NEMETH PM, 1988, J NEUROSCI, V8, P3959
[6] [DEOXYGLUCOSE-C-14 METHOD FOR MEASUREMENT OF LOCAL CEREBRAL GLUCOSE-UTILIZATION - THEORY, PROCEDURE, AND NORMAL VALUES IN CONSCIOUS AND ANESTHETIZED ALBINO-RAT
SOKOLOFF, L
REIVICH, M
KENNEDY, C
DESROSIERS, MH
PATLAK, CS
PETTIGREW, KD
SAKURADA, O
SHINOHARA, M
[J]. JOURNAL OF NEUROCHEMISTRY, 1977, 28 (05) : 897 - 916

← 1 →