CONTRIBUTION OF THE B-OLIGOMER TO THE PROTECTIVE ACTIVITY OF GENETICALLY ATTENUATED PERTUSSIS TOXIN

被引：11

作者：

ARCINIEGA, JL ^{[1
]}

SHAHIN, RD ^{[1
]}

BURNETTE, WN ^{[1
]}

BARTLEY, TD ^{[1
]}

WHITELEY, DW ^{[1
]}

MAR, VL ^{[1
]}

BURNS, DL ^{[1
]}

机构：

[1] AMGEN INC,THOUSAND OAKS,CA 91320

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 10期

关键词：

D O I：

10.1128/IAI.59.10.3407-3410.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An enzymatically deficient recombinant S1 subunit, in which Arg-9 was replaced by Lys, was combined with native B oligomer to form a mutant holotoxin molecule. This molecule exhibited decreased leukocytosis-promoting and histamine-sensitizing activities compared with those of the native toxin, supporting the view that the B oligomer is not responsible for these activities. The protective activity of this genetically attenuated pertussis toxin was compared with that of B oligomer alone. The mutant pertussis toxin and B oligomer were similarly capable of protecting mice against a respiratory infection with Bordetella pertussis, suggesting that the B oligomer makes a significant contribution to the protection afforded by the genetically attenuated holotoxin.

引用

页码：3407 / 3410

页数：4

共 26 条

[1] [Anonymous], 1988, LANCET, Vi, P955
[2] IMMUNE-RESPONSE TO THE B-OLIGOMER OF PERTUSSIS TOXIN
ARCINIEGA, JL
BURNS, DL
GARCIAORTIGOZA, E
MANCLARK, CR
[J]. INFECTION AND IMMUNITY, 1987, 55 (05) : 1132 - 1136
[3] PERTUSSIS HOLOTOXOID FORMED INVITRO WITH A GENETICALLY DEACTIVATED S1 SUBUNIT
BARTLEY, TD
WHITELEY, DW
MAR, VL
BURNS, DL
BURNETTE, WN
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) : 8353 - 8357
[4] MAPPING OF A PROTECTIVE EPITOPE OF PERTUSSIS TOXIN BY INVITRO REFOLDING OF RECOMBINANT FRAGMENTS
BARTOLONI, A
PIZZA, M
BIGIO, M
NUCCI, D
ASHWORTH, LA
IRONS, LI
ROBINSON, A
BURNS, D
MANCLARK, C
SATO, H
RAPPUOLI, R
[J]. BIO-TECHNOLOGY, 1988, 6 (06): : 709 - 712
[5] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6] Burnette W N, 1990, Biotechnology (N Y), V8, P1002, DOI 10.1038/nbt1190-1002
[7] PERTUSSIS TOXIN S1 MUTANT WITH REDUCED ENZYME-ACTIVITY AND A CONSERVED PROTECTIVE EPITOPE
BURNETTE, WN
CIEPLAK, W
MAR, VL
KALJOT, KT
SATO, H
KEITH, JM
[J]. SCIENCE, 1988, 242 (4875) : 72 - 74
[8] BURNS DL, 1987, J BIOL CHEM, V262, P17677
[9] Gabriel, 1971, METHOD ENZYMOL, V22, P565, DOI 10.1016/0076-6879(71)22041-3
[10] G-PROTEINS - TRANSDUCERS OF RECEPTOR-GENERATED SIGNALS
GILMAN, AG
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 : 615 - 649

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