STRUCTURAL ALTERATION IN ALTERNATING ADENINE-THYMINE SEQUENCES IN POSITIVELY SUPERCOILED DNA

被引：18

作者：

MCCLELLAN, JA ^{[1
]}

LILLEY, DMJ ^{[1
]}

机构：

[1] UNIV DUNDEE,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 02期

关键词：

DNA STRUCTURE; POSITIVE DNA SUPERCOILING; CHEMICAL REACTIVITY OF DNA; OSMIUM TETROXIDE; (A.T)N SEQUENCES;

D O I：

10.1016/0022-2836(91)90555-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An alternating adenine-thymine tract in a relaxed closed circular plasmid was found to become strongly reactive to osmium tetroxide in the presence of actinomycin D). We suggest that this is due to a local overwinding of the alternating tract as a result of positive supercoiling induced by intercalation of the antibiotic at GpC sequences elsewhere in the DNA. We have previously shown that (A·T)n sequences undergo a local underwinding in response to negative supercoiling, and it appears that such sequences are especially torsionally deformable in both directions. © 1991.

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页码：145 / 149

页数：5

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