A RECOMBINANT FOWLPOX VIRUS THAT EXPRESSES THE VP2 ANTIGEN OF INFECTIOUS BURSAL DISEASE VIRUS INDUCES PROTECTION AGAINST MORTALITY CAUSED BY THE VIRUS

被引：92

作者：

BAYLISS, CD ^{[1
]}

PETERS, RW ^{[1
]}

COOK, JKA ^{[1
]}

REECE, RL ^{[1
]}

HOWES, K ^{[1
]}

BINNS, MM ^{[1
]}

BOURSNELL, MEG ^{[1
]}

机构：

[1] AFRC,INST ANIM HLTH,HOUGHTON LAB,HOUGHTON PE17 2DA,HUNTS,ENGLAND

来源：

ARCHIVES OF VIROLOGY | 1991年 / 120卷 / 3-4期

关键词：

D O I：

10.1007/BF01310475

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The coding sequences of VP 2 from a virulent strain, 52/70, of infectious bursal disease virus (IBDV) were excised from a cDNA clone and inserted into a fowlpox plasmid insertion vector. The resulting plasmid, pIBD 1, was used to construct a recombinant fowlpox virus, fpIBD 1, which expressed VP 2 as a beta-galactosidase fusion protein. Chickens vaccinated with fpIBD 1 at 1 and 14 days of age, were challenged at 28 days with either IBDV strain 52/70 or the highly virulent strain CS 89. These chickens were protected against mortality, but not against damage to the bursa of Fabricius. The protection achieved by the use of fpIBD 1 shows that VP 2 is a host protective antigen.

引用

页码：193 / 205

页数：13

共 33 条

[1] DELETION MAPPING AND EXPRESSION IN ESCHERICHIA-COLI OF THE LARGE GENOMIC SEGMENT OF A BIRNAVIRUS [J].

AZAD, AA ;

JAGADISH, MN ;

BROWN, MA ;

HUDSON, PJ .

VIROLOGY, 1987, 161 (01) :145-152

[2] EXPRESSION IN ESCHERICHIA-COLI OF CDNA FRAGMENTS ENCODING THE GENE FOR THE HOST-PROTECTIVE ANTIGEN OF INFECTIOUS BURSAL DISEASE VIRUS [J].

AZAD, AA ;

FAHEY, KJ ;

BARRETT, SA ;

ERNY, KM ;

HUDSON, PJ .

VIROLOGY, 1986, 149 (02) :190-198

[3]

AZAD AA, 1988, Patent No. 8800206

[4] A COMPARISON OF THE SEQUENCES OF SEGMENT-A OF 4 INFECTIOUS BURSAL DISEASE VIRUS-STRAINS AND IDENTIFICATION OF A VARIABLE REGION IN VP2 [J].

BAYLISS, CD ;

SPIES, U ;

SHAW, K ;

PETERS, RW ;

PAPAGEORGIOU, A ;

MULLER, H ;

BOURSNELL, MEG .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :1303-1312

[5]

BAYLISS CD, 1990, THESIS U WALES ABERY

[6] COMPARATIVE STUDIES ON STRUCTURAL AND ANTIGENIC PROPERTIES OF 2 SEROTYPES OF INFECTIOUS BURSAL DISEASE VIRUS [J].

BECHT, H ;

MULLER, H ;

MULLER, HK .

JOURNAL OF GENERAL VIROLOGY, 1988, 69 :631-640

[7] IDENTIFICATION BY A RANDOM SEQUENCING STRATEGY OF THE FOWLPOXVIRUS DNA-POLYMERASE GENE, ITS NUCLEOTIDE-SEQUENCE AND COMPARISON WITH OTHER VIRAL-DNA POLYMERASES [J].

BINNS, MM ;

STENZLER, L ;

TOMLEY, FM ;

CAMPBELL, J ;

BOURSNELL, MEG .

NUCLEIC ACIDS RESEARCH, 1987, 15 (16) :6563-6573

[8] INSERTION OF THE FUSION GENE FROM NEWCASTLE-DISEASE VIRUS INTO A NONESSENTIAL REGION IN THE TERMINAL REPEATS OF FOWLPOX VIRUS AND DEMONSTRATION OF PROTECTIVE IMMUNITY INDUCED BY THE RECOMBINANT [J].

BOURSNELL, MEG ;

GREEN, PF ;

CAMPBELL, JIA ;

DEUTER, A ;

PETERS, RW ;

TOMLEY, FM ;

SAMSON, ACR ;

CHAMBERS, P ;

EMMERSON, PT ;

BINNS, MM .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :621-628

[9] A RECOMBINANT FOWLPOX VIRUS EXPRESSING THE HEMAGGLUTININ-NEURAMINIDASE GENE OF NEWCASTLE-DISEASE VIRUS (NDV) PROTECTS CHICKENS AGAINST CHALLENGE BY NDV [J].

BOURSNELL, MEG ;

GREEN, PF ;

SAMSON, ACR ;

CAMPBELL, JIA ;

DEUTER, A ;

PETERS, RW ;

MILLAR, NS ;

EMMERSON, PT ;

BINNS, MM .

VIROLOGY, 1990, 178 (01) :297-300

[10]

BOX P, 1989, POULTRY WORLD 1123

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