DEVICE TO PREPARE EXTRUDED NUCLEI AND CHROMOSOME SQUASHES

被引：7

作者：

BERRIOS, M

机构：

[1] Department of Pharmacological Sciences, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1994年 / 69卷 / 02期

基金：

美国国家科学基金会;

关键词：

DROSOPHILA; NUCLEUS; CHROMOSOMES; CYTOCHEMISTRY; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; MICROSCOPY;

D O I：

10.3109/10520299409106265

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A horizontal toggle clamp mounted on a rigid base plate makes nuclear extrusion and polytene chromosome squashing simple and reproducible. The base plate has a stage with shallow flat grooves to align the tissue sample directly below the clamp's swivel foot and hold the microscope slide in place during squashing. Appropriate pressure to obtain either extruded nuclei or squash preparations of polytene chromosomes is established empirically by adjusting the clamp's spindle assembly up or down.

引用

页码：78 / 80

页数：3

共 8 条

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[2] LOCALIZATION OF A MYOSIN HEAVY CHAIN-LIKE POLYPEPTIDE TO DROSOPHILA NUCLEAR-PORE COMPLEXES
BERRIOS, M
FISHER, PA
MATZ, EC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (01) : 219 - 223
[3] IDENTIFICATION AND CHARACTERIZATION OF A NUCLEAR-PORE COMPLEX PROTEIN
DAVIS, LI
BLOBEL, G
[J]. CELL, 1986, 45 (05) : 699 - 709
[4] MICRO-PRESS FOR CYTOLOGICAL PREPARATIONS
LARTER, EN
IKONEN, H
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[5] Pardue ML, 1986, DROSOPHILA PRACTICAL, P111
[6] POWELL JB, 1971, STAIN TECHNOL, V46, P211
[7] SCHMIDT T, 1985, J BIOL CHEM, V260, P7645
[8] SHIELDS G, 1975, J EMBRYOL EXP MORPH, V33, P159

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