THE KINETICS OF THE BETA-GALACTOSIDE-PROTON SYMPORT OF ESCHERICHIA-COLI

被引：35

作者：

PAGE, MGP

WEST, IC

机构：

来源：

BIOCHEMICAL JOURNAL | 1981年 / 196卷 / 03期

关键词：

D O I：

10.1042/bj1960721

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

.beta.-Galactoside transport by E. coli occurs with the concomitant uptake of a proton. The kinetics of .beta.-galactoside uptake at various values of external pH are interpreted in terms of a model in which both the galactoside and the proton are substrates of the transport reaction. The values of some of the kinetic constants for this 2-substrate reaction were determined. The observed effects of the proton motive force on the apparent Km for galactoside can be explained in terms of the proton being a substrate of the transport reaction.

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页码：721 / 731

页数：11

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