RIBOSOMAL PROTEINS .6. PREPARATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS AS APPLIED TO ISOLATION OF RIBOSOMAL PROTEINS

被引：30

作者：

KALTSCHMIDT, E

WITTMANN, HG

机构：

[1] Max-Planck-Institut für Molekulare Genetik, Berlin-Dahlem

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 30卷 / 01期

关键词：

D O I：

10.1016/0003-2697(69)90379-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new technique for the isolation of single proteins from complex protein mixtures by means of electrophoresis in polyacrylamide gels is described. This technique is also applicable to protein mixtures which are soluble only in urea. The method allows the resolution of multicomponent mixtures containing a variety of proteins which differ partly only slightly in electrophoretic mobility from each other. This system was used for the separation of ribosomal proteins of yeast and Escherichia coli. The capacity of the apparatus is 200-1000 mg of ribosomal proteins. © 1969.

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页码：132 / +

页数：1

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共 13 条

[1] RIBOSOMAL PROTEINS .4. ISOLATION AMINO ACID COMPOSITIONS PEPTIDE MAPS AND MOLECULAR WEIGHTS OF YEAST RIBOSOMAL PROTEINS [J].

CHERSI, A ;

DZIONARA, M ;

DONNER, D ;

WITTMANN, HG .

MOLECULAR AND GENERAL GENETICS, 1968, 101 (01) :82-&

[2] PREPARATIVE ZONE ELECTROPHORESIS OF PROTEINS ON POLYACRYLAMIDE GELS IN 8 M UREA [J].

DUESBERG, PH ;

RUECKERT, RR .

ANALYTICAL BIOCHEMISTRY, 1965, 11 (02) :342-&

[3] EXTRACTION AND ISOLATION OF INDIVIDUAL RIBOSOMAL PROTEINS FROM ESCHERICHIA COLI [J].

FOGEL, S ;

SYPHERD, PS .

JOURNAL OF BACTERIOLOGY, 1968, 96 (02) :358-&

[4] APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS [J].

JOVIN, T ;

NAUGHTON, MA ;

CHRAMBACH, A .

ANALYTICAL BIOCHEMISTRY, 1964, 9 (03) :351-&

[5] RIBOSOMAL PROTEINS .I. ISOLATION AMINO ACID COMPOSITION MOLECULAR WEIGHTS AND PEPTIDE MAPPING OF PROTEINS FROM E COLI RIBOSOMES [J].

KALTSCHMIDT, E ;

DZIONARA, M ;

DONNER, D ;

WITTMANN, HG .

MOLECULAR AND GENERAL GENETICS, 1967, 100 (04) :364-+

[6] PREPARATIVE METHODS FOR DISK ELECTROPHORESIS WITH SPECIAL REFERENCE TO ISOLATION OF PITUITARY HORMONES [J].

LEWIS, UJ ;

CLARK, MO .

ANALYTICAL BIOCHEMISTRY, 1963, 6 (04) :303-&

[7] PREPARATIVE ELECTROPHORESIS OF PROTEINS IN ACRYLAMIDE GELS [J].

MAIZEL, JV .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :382-&

[8]

ORNSTEIN L, 1962, DISC ELECTROPHORESIS

[9] PREPARATIVE ELECTROPHORESIS ON POLYACRYLAMIDE GEL [J].

RACUSEN, D ;

CALVANICO, N .

ANALYTICAL BIOCHEMISTRY, 1964, 7 (01) :62-+

[10] DISK ELECTROPHORESIS OF BASIC PROTEINS AND PEPTIDES ON POLYACRYLAMIDE GELS [J].

REISFELD, RA ;

LEWIS, UJ ;

WILLIAMS, DE .

NATURE, 1962, 195 (4838) :281-&