CALCIUM REQUIREMENT FOR THE PHOSPHATIDYLINOSITOL RESPONSE FOLLOWING ACTIVATION OF PRESYNAPTIC MUSCARINIC RECEPTORS

被引：57

作者：

GRIFFIN, HD

HAWTHORNE, JN

SYKES, M

ORLACCHIO, A

机构：

[1] UNIV NOTTINGHAM,HOSP & MED SCH,DEPT BIOCHEM,NOTTINGHAM NG7 2UH,ENGLAND

[2] UNIV PERUGIA,FAC PHARM,INST BIOL CHEM,I-06100 PERUGIA,ITALY

来源：

BIOCHEMICAL PHARMACOLOGY | 1979年 / 28卷 / 07期

关键词：

D O I：

10.1016/0006-2952(79)90320-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The labelling in vitro by [32P]phosphate of phosphatidic acid and phosphatidylinositol in synaptosomes from guinea-pig brain was studied. Acetylcholine increased the labelling and evidence is provided that pre-synaptic muscarinic receptors are involved. The increase was not seen in the presence of EGTA. Experiments with various calcium buffers indicated that concentrations of greater than 10-7 M free Ca2+ are required in the incubation medium for this phospholipid effect. A similar muscarinic effect in parotid gland is unaffected by EGTA. It is suggested that, in the parotid, postsynaptic receptors mediate increased phosphatidylinositol labelling and increased availability of Ca2+ for stimulus-secretion coupling. Presynaptic receptors similarly mediate increased labelling, which differs in being sensitive to EGTA and associated with decreased availability of Ca2+. © 1979.

引用

页码：1143 / 1147

页数：5

共 29 条

[1] ENZYMIC FORMATION OF MYOINOSITOL 1-2-CYCLIC PHOSPHATE FROM PHOSPHATIDYLINOSITOL [J].