PREPARATIVE PURIFICATION OF TETRABROMOTETRACHLOROFLUORESCEIN AND PHLOXINE-B BY CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY

被引：12

作者：

WEISZ, A ^{[1
]}

LANGOWSKI, AJ ^{[1
]}

MEYERS, MB ^{[1
]}

THIEKEN, MA ^{[1
]}

ITO, Y ^{[1
]}

机构：

[1] NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 538卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)91633-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A centrifugal counter-current chromatographic method for preparative purification of commercial tetrabromotetrachlorofluorescein and Phloxine B (D&C Red Nos. 27 and 28, respectively) was developed. Ethyl acetate-n-butanol-0.01 M ammonium acetate (1:1:2) was used as the two-phase solvent system. Each purification trial involved 50 mg of sample and yielded 22 mg (+/- 2 mg) of pure dye. The purity of the product was measured by high-performance liquid and thin-layer chromatography and was found to be 99.9%. The partition coefficients of these compounds were found to be highly concentration-dependent in the two-phase solvent system used. If this problem can be circumvented, then the counter-current chromatographic method can be extended for use with gram quantities of dye.

引用

页码：157 / 164

页数：8

共 16 条

[1] SYNTHESIS AND SPECTROSCOPIC PROPERTIES OF NEW ROSE-BENGAL AND EOSIN-Y DERIVATIVES [J].