EXPRESSION, IDENTIFICATION AND SUBCELLULAR-LOCALIZATION OF THE PROTEINS ENCODED BY THE HEPATITIS-C VIRAL GENOME

被引：206

作者：

SELBY, MJ ^{[1
]}

CHOO, QL ^{[1
]}

BERGER, K ^{[1
]}

KUO, G ^{[1
]}

GLAZER, E ^{[1
]}

ECKART, M ^{[1
]}

LEE, C ^{[1
]}

CHIEN, D ^{[1
]}

KUO, C ^{[1
]}

HOUGHTON, M ^{[1
]}

机构：

[1] CHIRON CORP,4560 HORTON ST,EMERYVILLE,CA 94608

来源：

JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷

关键词：

D O I：

10.1099/0022-1317-74-6-1103

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have expressed the full-length coding region and selected domains of the hepatitis C virus (HCV) cDNA in mammalian cells by transfection. Using HCV antibody-positive human sera and monospecific antibodies the proteins encoded by the putative structural and non-structural regions of the open reading frame of HCV were identified as core (p22), E1 (gp32-35), E2 (gp68-72), NS2 (p23), NS3 (p72), NS4a and b (p10 and p27) and NS5a and b (p56 and p70). We have also defined the subcellular localizations of the HCV proteins using indirect immunofluorescence assays.

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页码：1103 / 1113

页数：11

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