SIMULTANEOUS RADIOENZYMATIC ASSAY OF CATECHOLAMINES AND DIHYDROXY-PHENYLACETIC ACID DOPAC - COMPARISON OF EFFECTS OF DRUGS ON TUBEROINFUNDIBULAR AND STRIATAL DOPAMINE METABOLISM AND ON PLASMA PROLACTIN LEVEL

被引：55

作者：

FEKETE, MIK ^{[1
]}

KANYICSKA, B ^{[1
]}

HERMAN, JP ^{[1
]}

机构：

[1] INST ANIM HUSBANDRY,HERCEGHALOM,HUNGARY

来源：

LIFE SCIENCES | 1978年 / 23卷 / 15期

关键词：

D O I：

10.1016/0024-3205(78)90582-9

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A simultaneous radioenzymatic assay for catecholamines and DOPAC has been developed. 3H-Methoxy amines and 3H-homovanillic acid formed in the presence of 3H-S-adenosyl-methionine and catechol-O-methyl transferase were separated by organic solvent extraction and chromatography. Chlorpromazine, haloperidol, but not sulpiride increased the DOPAC content in the striatum, without affecting the DOPAC level in the median eminence. γ-Butyrolactone increased the dopamine content also only in the striatum. The monoamine oxidase inhibitor pargyline decreased the DOPAC level in both areas examined. All the substances employed increased plasma prolactin level. The biochemical responses of nigrostriatal and tubero-infundibular dopaminergic neurons may not be analogous. © 1978.

引用

页码：1549 / 1556

页数：8

共 23 条

[1] EFFECTS OF CHLORPROMAZINE, HALOPERIDOL AND RESERPINE ON THE LEVELS OF PHENOLIC ACIDS IN RABBIT CORPUS STRIATUM [J].