DETECTION OF N-ACETYLCYSTEINE, CYSTEINE AND THEIR DISULFIDES IN URINE BY LIQUID-CHROMATOGRAPHY WITH A DUAL-ELECTRODE AMPEROMETRIC DETECTOR

被引：14

作者：

STENKEN, JA ^{[1
]}

PUCKETT, DL ^{[1
]}

LUNTE, SM ^{[1
]}

LUNTE, CE ^{[1
]}

机构：

[1] UNIV KANSAS,DEPT CHEM,LAWRENCE,KS 66045

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 01期

关键词：

cysteine; dual-electrode detection; liquid chromatography/electrochemistry; N-Acetylcysteine;

D O I：

10.1016/0731-7085(90)80011-D

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of N-acetylcysteine, cysteine and their disulfides in urine is described. The thiols and disulfides are separated by reversed-phase ion-pair chromatography with octyl sodium sulfate as the ion-pairing reagent and detected with a dual-electrode amperometric detector using Au/Hg amalgam electrodes. Both the thiols and disulfides are detected with this system. In addition, dimers and mixed disulfides can be detected individually. © 1990.

引用

页码：85 / 89

页数：5

共 23 条

[1] SPECTROPHOTOMETRIC ASSAY FOR SULFHYDRYL GROUPS USING N-ETHYLMALEIMIDE [J].