THE USE OF FLUORESCENT-PROBES IN IMMUNOCHEMISTRY

被引：48

作者：

DAVIDSON, RS

HILCHENBACH, MM

机构：

[1] Department of Chemistry, City University, London, EC1V OHB, Northampton Square

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1990年 / 52卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1990.tb04200.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The limitations and advantages of particular dyes for labelling proteins and other biological materials are discussed. Methods available for conjugating dyes to proteins are outlined. Following a discussion of double labelling methods the use of photoactivatable fluorochromes and time resolved fluorescence methodologies are outlined. The reasons for the photoinstability of some fluorochromes are discussed and methods for overcoming the problem are described. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：431 / 438

页数：8

共 29 条

[1] ENERGY-TRANSFER LUMINESCENCE OF EUROPIUM(III) AND TERBIUM(III) CRYPTATES OF MACROBICYCLIC POLYPYRIDINE LIGANDS
ALPHA, B
LEHN, JM
MATHIS, G
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1987, 26 (03): : 266 - 267
[2] 2ND-SPHERE PHOTOCHEMISTRY AND PHOTOPHYSICS OF COORDINATION-COMPOUNDS
BALZANI, V
SABBATINI, N
SCANDOLA, F
[J]. CHEMICAL REVIEWS, 1986, 86 (02) : 319 - 337
[3] BATHOPHENANTHROLINE-RUTHENIUM(II) COMPLEXES AS NON-RADIOACTIVE LABELS FOR OLIGONUCLEOTIDES WHICH CAN BE MEASURED BY TIME-RESOLVED FLUORESCENCE TECHNIQUES
BANNWARTH, W
SCHMIDT, D
STALLARD, RL
HORNUNG, C
KNORR, R
MULLER, F
[J]. HELVETICA CHIMICA ACTA, 1988, 71 (08) : 2085 - 2099
[4] BANNWARTH W, 1989, TETRAHEDRON LETT, P1513
[5] BARNARD GJR, 1988, COMPLEMENTARY IMMUNO, P149
[6] BRADBURY S J, 1989, P187
[7] FLUORESCENCE QUENCHING OF FLUORESCEIN BY R-PHYCOERYTHRIN - A PITFALL IN DUAL FLUORESCENCE ANALYSIS
CHAPPLE, MR
JOHNSON, GD
DAVIDSON, RS
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 111 (02) : 209 - 217
[8] PHOTOACTIVABLE FLUOROPHORES .1. SYNTHESIS AND PHOTOACTIVATION OF ORTHO-NITROBENZYL-QUENCHED FLUORESCENT CARBAMATES
CUMMINGS, RT
KRAFFT, GA
[J]. TETRAHEDRON LETTERS, 1988, 29 (01) : 65 - 68
[9] PHOTOACTIVABLE FLUOROPHORES .2. SYNTHESIS AND PHOTOACTIVATION OF FUNCTIONALIZED 3-AROYL-2-(2-FURYL)-CHROMONES
CUMMINGS, RT
DIZIO, JP
KRAFFT, GA
[J]. TETRAHEDRON LETTERS, 1988, 29 (01) : 69 - 72
[10] THE EFFECTS OF ENZYMES ON THE PHOTOBLEACHING OF FLUORESCEIN AND FLUORESCEIN ISOTHIOCYANATE CONJUGATES
DAVIDSON, RS
PRATT, JE
[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1988, 1 (03) : 361 - 369

← 1 2 3 →