VACCINATION AGAINST TAENIA-TAENIAEFORMIS INFECTION IN RATS USING A RECOMBINANT PROTEIN AND PRELIMINARY-ANALYSIS OF THE INDUCED ANTIBODY-RESPONSE

被引：26

作者：

ITO, A

BOGH, HO

LIGHTOWLERS, MW

MITCHELL, GF

TAKAMI, T

KAMIYA, M

ONITAKE, K

RICKARD, MD

机构：

[1] CSIRO,DIV ANIM HLTH,MELBOURNE,VIC 3001,AUSTRALIA

[2] WALTER & ELIZA HALL INST MED RES,MELBOURNE,AUSTRALIA

[3] HOKKAIDO UNIV,FAC VET MED,DEPT PARASITOL,SAPPORO,HOKKAIDO 060,JAPAN

[4] GIFU UNIV,SCH MED,DEPT PATHOL,GIFU 500,JAPAN

[5] UNIV MELBOURNE,CTR VET CLIN,WERRIBEE,VIC 3030,AUSTRALIA

[6] YAMAGATA UNIV,FAC SCI,DEPT BIOL,YAMAGATA 990,JAPAN

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 44卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0166-6851(91)90219-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primary screening of a cDNA expression library of Taenia taeniaeformis oncospheres in λgt11 bacteriophage was carried out using rabbit anti-T. taeniaeformis oncosphere serum affinity-purified from disrupted oncosphere pellets. From approximately 1.6 × 105 plaques, 21 single clones that were positive with the affinity-purified antibodies were isolated. Sibling analysis revealed that 17 clones out of the 21 could be assigned to five different antigen families. Only family 1 was strongly recognized by a serum prepared in a rabbit against a partially purified host-protective oncosphere antigen fraction. The fragments of λDNA were inserted into a pGEX plasmid vector that encodes glutathione S-transferase (GST) of Schistosoma japonicum. Clones designated TtO-18, -49, 53 (family 1), 46 (family 2), 15 (family 3), 40 (family 4) and 66 (family 5) were established as subclones in pGEX-1 plasmid vectors which produced GST fusion proteins. All GST fusion proteins were soluble and recognized by anti-GST and anti-TtO sera. Three vaccination experiments with these fusion proteins using specific-pathogen-free Wistar rats revealed that all three fusion proteins of family 1 were exclusively effective against T. taeniaeformis oncosphere challenge with approximately 95% and 91% reductions in cystic metacestode and total metacestode recoveries, respectively. Rats vaccinated with fusion proteins of family 1 produced antibodies which reacted with a 21-kDa oncosphere antigen component which appeared to be a major oncosphere stage-specific antigen. © 1991.

引用

页码：43 / 49

页数：7

共 24 条

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