ALLELE-SPECIFIC POLYMERASE CHAIN-REACTION - A METHOD FOR AMPLIFICATION AND SEQUENCE DETERMINATION OF A SINGLE COMPONENT AMONG A MIXTURE OF SEQUENCE VARIANTS

被引：168

作者：

SUZUKI, Y ^{[1
]}

SEKIYA, T ^{[1
]}

HAYASHI, K ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV ONCOGENE,1-1 TSUKIJI 5 CHOME,CHUO KU,TOKYO 104,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90188-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new technique is described for amplifying individual alleles in a mixture of two or more alleles by the polymerase chain reaction (PCR) to determine their nucleotide sequence. This technique involves amplifying and separating target sequences by the PCR-mediated single-strand conformation polymorphism (PCR-SSCP) method, isolating each polymorphic DNA strand, and amplifying it by a second-stage PCR for its sequence determination. By this technique, the sequence of a minor constituent (approximately 3%) can be determined accurately. © 1991.

引用

页码：82 / 84

页数：3

共 16 条

[1] GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES [J].

BLIN, N ;

STAFFORD, DW .

NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2303-2308

[2]

COLLINS SJ, 1989, BLOOD, V73, P1028

[3] DETECTION OF SICKLE-CELL BETA-S-GLOBIN ALLELE BY HYBRIDIZATION WITH SYNTHETIC OLIGONUCLEOTIDES [J].

CONNER, BJ ;

REYES, AA ;

MORIN, C ;

ITAKURA, K ;

TEPLITZ, RL ;

WALLACE, RB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :278-282

[4]

COTTON RGH, 1989, BIOCHEM J, V263, P1

[5] DNA FRAGMENTS DIFFERING BY SINGLE BASE-PAIR SUBSTITUTIONS ARE SEPARATED IN DENATURING GRADIENT GELS - CORRESPONDENCE WITH MELTING THEORY [J].

FISCHER, SG ;

LERMAN, LS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (06) :1579-1583

[6] GENERATION OF SINGLE-STRANDED-DNA BY THE POLYMERASE CHAIN-REACTION AND ITS APPLICATION TO DIRECT SEQUENCING OF THE HLA-DQA LOCUS [J].

GYLLENSTEN, UB ;

ERLICH, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7652-7656

[7] USE OF LABELED PRIMERS IN POLYMERASE CHAIN-REACTION (LP-PCR) FOR A RAPID DETECTION OF THE PRODUCT [J].

HAYASHI, K ;

ORITA, M ;

SUZUKI, Y ;

SEKIYA, T .

NUCLEIC ACIDS RESEARCH, 1989, 17 (09) :3605-3605

[8]

MACGRATH JP, 1983, NATURE, V304, P501

[9] DETECTION OF POLYMORPHISMS OF HUMAN DNA BY GEL-ELECTROPHORESIS AS SINGLE-STRAND CONFORMATION POLYMORPHISMS [J].

ORITA, M ;

IWAHANA, H ;

KANAZAWA, H ;

HAYASHI, K ;

SEKIYA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2766-2770

[10] RAPID AND SENSITIVE DETECTION OF POINT MUTATIONS AND DNA POLYMORPHISMS USING THE POLYMERASE CHAIN-REACTION [J].

ORITA, M ;

SUZUKI, Y ;

SEKIYA, T ;

HAYASHI, K .

GENOMICS, 1989, 5 (04) :874-879

← 1 2 →