IDENTIFICATION OF ORAL YEASTS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：8

作者：

MAIDEN, MFJ

TANNER, A

机构：

[1] Forsyth Dental Center, Boston, Massachusetts

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 03期

关键词：

ORAL YEAST; CANDIDA-ALBICANS; SDS-PAGE; IDENTIFICATION;

D O I：

10.1111/j.1399-302X.1991.tb00475.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Protein profiles of sonicated cells for 9 species of yeasts isolated from oral samples were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and visualized with a silver stain. The profiles obtained on 12% acrylamide gels were distinct, and characteristic for 6 species of Candida, and Torulopsis glabrata, Yarrowia lipolytica, and Saccharomyces cerevisiae. Using this method, 79 fresh isolates of yeasts from saliva samples were identified; 58 as Candida albicans, 9 as Candida parapsilosis, 1 as Candida tropicalis, and 11 as T. glabrata. SDS-PAGE offers a rapid, convenient alternative or adjunct to yeast identification systems based on carbohydrate assimilation tests.

引用

页码：187 / 190

页数：4

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