Hybridization of DNA targets to glass-tethered oligonucleotide probes

被引：48

作者：

Beattie, WG ^{[1
]}

Meng, L ^{[1
]}

Turner, SL ^{[1
]}

Varma, RS ^{[1
]}

Dao, DD ^{[1
]}

Beattie, KL ^{[1
]}

机构：

[1] HOUSTON ADV RES CTR,DNA TECHNOL LAB,THE WOODLANDS,TX 77381

来源：

MOLECULAR BIOTECHNOLOGY | 1995年 / 4卷 / 03期

关键词：

genosensors; sequencing by hybridization; oligonucleotide arrays; DNA chips; genome mapping; DNA sequencing; DNA probes; mutation detection;

D O I：

10.1007/BF02779015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydridization of nucleic acids to surface-tethered oligonucleotide probes has numerous potential applications in genome mapping and DNA sequence analysis. In this article, we describe a simple standard protocol for routine preparation of terminal amine-derivatized 9-mer oligonucleotide arrays on ordinary microscope slides and hybridization conditions with DNA target strands of up to several hundred bases in length with good discrimination against mismatches. Additional linker arms separating the glass surface from the probe sequence are not necessary. The technique described here offers a powerful tool for the detection of specific genetic mutations.

引用

页码：213 / 225

页数：13

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