THE PROTEIN EFFECT ON DETERMINATION OF DNA WITH HOECHST-33258

被引：16

作者：

MOE, D

GARBARSCH, C

KIRKEBY, S

机构：

[1] UNIV COPENHAGEN,PANUM INST,FAC HLTH SCI,SCH DENT,DEPT MICROBIOL,DK-2200 COPENHAGEN N,DENMARK

[2] UNIV COPENHAGEN,PANUM INST,FAC HLTH SCI,INST MED ANAT,DEPT A,DK-2200 COPENHAGEN,DENMARK

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1994年 / 28卷 / 04期

关键词：

DNA METHOD; HOECHST; 33258; PROTEIN INTERFERENCE;

D O I：

10.1016/0165-022X(94)90002-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was designed to afford a critical review of the effect of proteins on the Hoechst 33258 method for determination of DNA of crude homogenates. A considerable effect of proteins on the fluorescence was observed when the concentration exceeded 100 mu g BSA equivalent protein. Below that value, practically no effect of proteins was noted. We used proteinase K to remove the proteins, but dilution of homogenates could be used as well. Moreover, we found that the concentration of the fluorochrome should be between 1 mu g and 2 mu g when microgram levels DNA are to be determined.

引用

页码：263 / 276

页数：14

共 34 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].