THE RGD CONTAINING SITE OF THE MOUSE LAMININ A CHAIN IS ACTIVE FOR CELL ATTACHMENT, SPREADING, MIGRATION AND NEURITE OUTGROWTH

被引：109

作者：

TASHIRO, K ^{[1
]}

SEPHEL, GC ^{[1
]}

GREATOREX, D ^{[1
]}

SASAKI, M ^{[1
]}

SHIRASHI, N ^{[1
]}

MARTIN, GR ^{[1
]}

KLEINMAN, HK ^{[1
]}

YAMADA, Y ^{[1
]}

机构：

[1] NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1991年 / 146卷 / 03期

关键词：

D O I：

10.1002/jcp.1041460316

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The laminin A chain has been sequenced by cDNA cloning and was found to contain an RGD sequence. Synthetic peptides containing the RGD sequence and flanking amino acids were active in mediating cell adhesion, spreading, migration, and neurite outgrowth. Furthermore, endothelial cell attachment to a laminin substrate was inhibited by an RGD-containing synthetic peptide. Antisera against the integrin (fibronectin) receptor, and monoclonal antibody to the integrin, VLA-6, inhibited cell interaction with laminin, as well as with peptides containing an RGD sequence. These results suggest that the RGD containing site of laminin is active and interacts with the integrin family of receptors in certain cells.

引用

页码：451 / 459

页数：9

共 58 条

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