AUTORADIOGRAPHIC STUDY OF IRON-BINDING SITES IN THE RAT-BRAIN - DISTRIBUTION AND RELATIONSHIP TO AGING

Specific binding of Fe-59 to various brain structures was investigated in rats using nanomolar concentrations of (FeCl3)-Fe-59 and quantitative autoradiography. Saturation studies revealed high affinity binding (k(d) in the nanomolar range) with binding sites density (B(max)) which varied in different brain regions from 462 fmol per mg tissue in the central thalamic nuclei to over 4 pmol per mg tissue in the cerebral peduncle. Binding was seen in both white and gray matter structures. B(max) values for frontal cortex, dentate gyrus, and substantia nigra were significantly lower in older rats. The distribution of Fe-59 binding sites was not consistent with the distribution of brain iron as reported by other investigators. Fe-59 binding was reduced significantly in the presence of free radicals. These observations suggest that high affinity binding sites for iron are localized differentially in various brain structures and may play an important role in the translocation and storage of potentially harmful ferric cations in brain. The finding that the capacity of the brain tissue to bind iron diminished with age in discrete brain regions suggests that in the aged animal, the removal of "free" iron from the cellular domain may be impaired in such regions, leading to increased susceptibility to iron-enhanced lipid peroxidation and cell death.