HPLC SEPARATION OF CATECHOLAMINES AFTER DERIVATIZATION WITH 9-FLUORENYLMETHYL CHLOROFORMATE

被引：9

作者：

DESCOMBES, AA ^{[1
]}

HAERDI, W ^{[1
]}

机构：

[1] UNIV GENEVA,DEPT INORGAN ANALYT & APPL CHEM,30 QUAI ERNEST ANSERMET,CH-1211 GENEVA 4,SWITZERLAND

来源：

CHROMATOGRAPHIA | 1992年 / 33卷 / 1-2期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; PRE-DERIVATIZATION; CATECHOLAMINES; 9-FLUORENYLMETHYL CHLOROFORMATE (FMOC-CL);

D O I：

10.1007/BF02276857

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimum conditions for the separation of 9-fluorenylmethyl chloroformate derivatized catecholamines by HPLC are described; three catecholamines (noradrenaline, adrenaline and dopamine) and an internal standard (epinine) were separated in less than 20 minutes under isocratic conditions. This method is 17 to 350 times more sensitive than electrochemical detection, depending on the test compounds. It has been applied to the analysis of catecholamines in urine. The sample was extracted by a metal-loaded silica prior to separation.

引用

页码：83 / 86

页数：4

共 17 条

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