CHANGES IN THE EXPRESSION OF LECTINS IN HUMAN LYMPHOCYTE-T MEMBRANE UPON MITOGENIC STIMULATION

被引:8
作者
GRILLON, C
MONSIGNY, M
KIEDA, C
机构
[1] CNRS, CTR BIOPHYS MOLEC, DEPT BIOCHIM GLYCOCONJUGUES & LECTINES ENDOGENES, F-45071 ORLEANS, FRANCE
[2] UNIV ORLEANS, F-45071 ORLEANS, FRANCE
关键词
D O I
10.1016/S0008-6215(00)90615-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Surface lectins, specific for given sugar structures, are expressed on human T cells, as shown by flow cytofluorometry using F-neoglycoproteins bearing either beta- and alpha-D-galactosyl, beta-D-galactosyl 6-phosphate, or alpha-L-rhamnosyl groups, but not by F-neoglycoproteins bearing other sugar groups (such as alpha-D-mannosyl groups). After stimulation with Phaseolus vulgaris mitogen, the number of cells that bind beta-D-galactosyl 6-phosphate groups (6-P-beta-D-Galp lec+ cells) increased fourfold during the first five days; these cells are helper (CD4+) T cells. Conversely, cells that bind alpha-L-Rha groups belong to the T suppressor (CD8+) family and their number moderately increased. Upon stimulation by concanavalin A, the number of cells expressing the lectin recognizing alpha-L-Rha groups increased during the first two days and then decreased within the next two days. These results are discussed with regard to the implication of lymphocyte membrane lectins in the suppressor mechanism and in the homing process.
引用
收藏
页码:283 / 292
页数:10
相关论文
共 47 条
[1]  
ACKERMAN SK, 1978, J IMMUNOL, V120, P1372
[2]  
AGPAR JR, 1982, EUR J IMMUNOL, V12, P570
[3]   HUMAN RECOMBINANT GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR INCREASES CELL-TO-CELL ADHESION AND SURFACE EXPRESSION OF ADHESION-PROMOTING SURFACE GLYCOPROTEINS ON MATURE GRANULOCYTES [J].
ARNAOUT, MA ;
WANG, EA ;
CLARK, SC ;
SIEFF, CA .
JOURNAL OF CLINICAL INVESTIGATION, 1986, 78 (02) :597-601
[4]   CARBOHYDRATE-SPECIFIC RECEPTORS OF THE LIVER [J].
ASHWELL, G ;
HARFORD, J .
ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :531-554
[5]  
AUNE TM, 1984, LYMPHOKINES, V9, P257
[6]  
BARZILAY M, 1982, LECTINS BIOL BIOCH C, V2, P67
[7]  
BENTWICH Z, 1973, CLIN IMMUNOL IMMUNOP, V1, P511, DOI 10.1016/0090-1229(73)90007-X
[8]   ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - AN INDUCIBLE RECEPTOR FOR NEUTROPHILS RELATED TO COMPLEMENT REGULATORY PROTEINS AND LECTINS [J].
BEVILACQUA, MP ;
STENGELIN, S ;
GIMBRONE, MA ;
SEED, B .
SCIENCE, 1989, 243 (4895) :1160-1165
[9]  
BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P77
[10]   CELL-SURFACE CARBOHYDRATES IN CELL RECOGNITION AND RESPONSE [J].
BRANDLEY, BK ;
SCHNAAR, RL .
JOURNAL OF LEUKOCYTE BIOLOGY, 1986, 40 (01) :97-111