STRUCTURE OF A RHOMBOHEDRAL R6 INSULIN PHENOL COMPLEX

被引：57

作者：

SMITH, GD ^{[1
]}

DODSON, GG ^{[1
]}

机构：

[1] UNIV YORK,YORK YO1 5DD,N YORKSHIRE,ENGLAND

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1992年 / 14卷 / 03期

关键词：

X-RAY DIFFRACTION; ALLOSTERISM; ZINC PHENOL INTERACTION;

D O I：

10.1002/prot.340140309

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hexameric insulin has been crystallized from different conditions in a variety of crystalline modifications. In the presence of approximately 1% phenol and at a pH of 8.5, a new rhombohedral form is produced, space group R3, a = 79.92angstrom and c = 40.39angstrom, in which the asymmetric unit consists of a dimer. The structure has been solved and refined, using data between 8.0 and 2.5angstrom resolution, to a residual of 0.157. The two monomers in the asymmetric unit have nearly identical R conformations, that is, residues BI through B8 are alpha-helical, producing a continuous alpha-helix from B1 through B19. A phenol molecule is hydrogen bonded to the carbonyl oxygen of A6 Cys of each monomer. Small differences in conformation and the final (2F(o)-F(c)) and difference electron density maps suggest that an additional phenol molecule is coordinated to one of the two zinc ions.

引用

页码：401 / 408

页数：8

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