THE ROLE OF PILI IN THE ATTACHMENT OF PSEUDOMONAS-AERUGINOSA TO UNWORN HYDROGEL CONTACT-LENSES

被引:24
作者
FLETCHER, EL
WEISSMAN, BA
EFRON, N
FLEISZIG, SMJ
CURCIO, AJ
BRENNAN, NA
机构
[1] Corneal Biophysics Laboratory, Department of Optometry, University of Melbourne, Parkville, VIC
[2] Jules Stein Eye Institute, Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA
[3] Department of Optometry and Vision Sciences, University of Manchester Institute of Science and Technology, Manchester
[4] Channing Laboratory, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
关键词
PSEUDOMONAS AERUGINOSA; CORNEA; CONTACT LENS; INFECTIVE KERATITIS; BACTERIAL ADHERENCE; PILL;
D O I
10.3109/02713689309033504
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Contamination of contact lenses is thought to increase the risk of infectious keratitis, yet factors promoting attachment of bacteria to contact lenses are not fully understood. It has been suggested that strains of Pseudomonas aeruginosa attach to mucosal surfaces via pill which are appendages found on some strains. This study investigated the role of pill and the effect of incubation time on the attachment of P. aeruginosa to 20 unworn hydrogel lenses representative of each of the four FDA categories. Ten lenses were incubated for 15 minutes and another ten for 180 minutes. Lenses were incubated with either PAK+ P. aeruginosa which possessed pill or its isogenic mutant pair, PAK-, which was genetically similar except for the absence of pill. Bacteria were quantified, following homogenization of the contact lens, by viable counts. Non-piliated bacteria were significantly more likely to adhere to the lenses (p < 0.001). A significant interaction between lens type and incubation time was observed (p < 0.05); thus it is difficult to generalize about either of these effects in isolation. These results show that surface characteristics may confer an attachment advantage to bacteria.
引用
收藏
页码:1067 / 1071
页数:5
相关论文
共 39 条
[1]   ULCERATIVE KERATITIS ASSOCIATED WITH CONTACT-LENS WEAR [J].
ALFONSO, E ;
MANDELBAUM, S ;
FOX, MJ ;
FORSTER, RK .
AMERICAN JOURNAL OF OPHTHALMOLOGY, 1986, 101 (04) :429-433
[2]   EFFECT OF LID CLOSURE ON CONTACT LENS-ASSOCIATED PSEUDOMONAS KERATITIS [J].
ASWAD, MI ;
BARZA, M ;
BAUM, J .
ARCHIVES OF OPHTHALMOLOGY, 1989, 107 (11) :1667-1670
[3]   ATTACHMENT OF PSEUDOMONAS TO HUMAN-WORN, DISPOSABLE ETAFILCON-A CONTACT-LENSES [J].
BOLES, SF ;
REFOJO, MF ;
LEONG, FL .
CORNEA, 1992, 11 (01) :47-52
[4]  
BOLES SF, 1991, INVEST OPHTH VIS SCI, V32, P729
[5]  
BUTRUS SI, 1987, OPHTHALMOLOGY, V94, P1310
[6]   CONTACT-LENS SURFACE DEPOSITS INCREASE THE ADHESION OF PSEUDOMONAS-AERUGINOSA [J].
BUTRUS, SI ;
KLOTZ, SA .
CURRENT EYE RESEARCH, 1990, 9 (08) :717-724
[7]   MUCOID PSEUDOMONAS-AERUGINOSA RESISTS NONOPSONIC PHAGOCYTOSIS BY HUMAN-NEUTROPHILS AND MACROPHAGES [J].
CABRAL, DA ;
LOH, BA ;
SPEERT, DP .
PEDIATRIC RESEARCH, 1987, 22 (04) :429-431
[8]   ROLE OF LIPOPOLYSACCHARIDE IN VIRULENCE OF PSEUDOMONAS-AERUGINOSA [J].
CRYZ, SJ ;
PITT, TL ;
FURER, E ;
GERMANIER, R .
INFECTION AND IMMUNITY, 1984, 44 (02) :508-513
[9]  
DOIG P, 1988, INFECT IMMUN, V56, P1641, DOI 10.1128/IAI.56.6.1641-1646.1988
[10]  
DOIG P, 1990, INFECT IMMUN, V58, P124, DOI 10.1128/IAI.58.1.124-130.1990