LEISHMANIA-SPP - EFFECT OF INHIBITORS ON GROWTH AND ON POLYAMINE AND MACROMOLECULAR SYNTHESES

被引：35

作者：

BACHRACH, U ^{[1
]}

BREM, S ^{[1
]}

WERTMAN, SB ^{[1
]}

SCHNUR, LF ^{[1
]}

GREENBLATT, CL ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH HOSP & MED SCH,CTR STUDY INFECT & TROP DIS,DEPT PROTOZOOL,JERUSALEM,ISRAEL

来源：

EXPERIMENTAL PARASITOLOGY | 1979年 / 48卷 / 03期

关键词：

Culture; Ethidium bromide; Growth cycle; Hemoflagellates; Inhibitors; Leishmania donovani Protozoa; parasitic; Leishmania mexicana; Leishmania spp; Leishmania tropica major; MGBG [methylglyoxal-bis (guanylhydrazone); Pentamidine; Polyamines; Putrescine; Spermidine; Spermine;

D O I：

10.1016/0014-4894(79)90131-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Ethidium bromide, pentamidine isethionate, and MGBG [methylglyoxal-bis (guanylhydrazone)] inhibited the uptake of radioactive putrescine by leishmanial (Leishmania spp.; Leishmania tropica major; Leishmania mexicana; Leishmania donovani) promastigotes and interfered with their polyamine synthesis. Inhibition was apparent as early as 1 hr after adding these drugs to the parasites at growth-inhibiting concentrations. Ethidium bromide also inhibited the incorporation of radioactive uracil into leishmanial RNA at growth-inhibiting concentrations, while DNA synthesis was inhibited by ethidium bromide at high concentrations after a lag period. MGBG inhibited the synthesis of leishmanial DNA and RNA at growth-inhibiting concentrations. © 1979.

引用

页码：464 / 470

页数：7

共 14 条

[1] THERAPY OF LEISHMANIASIS - SUPERIOR EFFICACIES OF LIPOSOME-ENCAPSULATED DRUGS
ALVING, CR
STECK, EA
CHAPMAN, WL
WAITS, VB
HENDRICKS, LD
SWARTZ, GM
HANSON, WL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (06) : 2959 - 2963
[2] LEISHMANIA-SPP - CELLULAR-LEVELS AND SYNTHESIS OF POLYAMINES DURING GROWTH CYCLES
BACHRACH, U
BREM, S
WERTMAN, SB
SCHNUR, LF
GREENBLATT, CL
[J]. EXPERIMENTAL PARASITOLOGY, 1979, 48 (03) : 457 - 463
[3] A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER
BRAY, GA
[J]. ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) : 279 - 285
[4] BRUN R, 1978, ACTA TROP, V35, P239
[5] EFFECTS OF METHYLGLYOXAL BIS(GUANYLHYDRAZONE) ON TRYPANOSOMATID FLAGELLATES - INHIBITION OF GROWTH AND NUCLEOSIDE INCORPORATION IN TRYPANOSOMA-BRUCEI
CHANG, KP
STEIGER, RF
DAVE, C
CHENG, YC
[J]. JOURNAL OF PROTOZOOLOGY, 1978, 25 (01): : 145 - 149
[6] ORNITHINE DECARBOXYLASE MAY FUNCTION AS AN INITIATION-FACTOR FOR RNA POLYMERASE-I
MANEN, CA
RUSSELL, DH
[J]. SCIENCE, 1977, 195 (4277) : 505 - 506
[7] MIHICH E, 1963, CANCER RES, V23, P1375
[8] NEWTON B. A., 1966, SYM SOC GEN MICROBIOL, V16, P213
[9] REACTIONS OF QUININE CHLOROQUINE AND QUINACRINE WITH DNA AND THEIR EFFECTS ON DNA AND RNA POLYMERASE REACTIONS
OBRIEN, RL
OLENICK, JG
HAHN, FE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (06) : 1511 - &
[10] BINDING OF POLYAMINES AND OF ETHIDIUM-BROMIDE TO TRANSFER-RNA
SAKAI, TT
TORGET, R
I, J
FREDA, CE
COHEN, SS
[J]. NUCLEIC ACIDS RESEARCH, 1975, 2 (07) : 1005 - 1022

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