TYPE-II PHOSPHOLIPASE-A2 RECOMBINANT OVEREXPRESSION ENHANCES STIMULATED ARACHIDONIC-ACID RELEASE

被引：81

作者：

PERNAS, P

MASLIAH, J

OLIVIER, JL

SALVAT, C

RYBKINE, T

BEREZIAT, G

机构：

[1] URA CNRS 1283, Laboratoire de Biochimie CHU Saint Antoine, Université Pierre et Marie Curie -, 75571 Paris Cedex 12, 27, rue Chaligny

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91035-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coding sequence of type II phospholipase A2 from human placenta was cloned in a bovine papilloma virus-derived eukaryotic expression vector under the control of the metallothionein promoter. Stably transfected C127 mouse fibroblast lines were obtained with this vector. These transfected cells overexpressed a functional 14 kDa phospholipase A2, which was bulky secreted. However, a significant phospholipase A2 activity was measured in cell homogenates. The involvement of this 14 kDa phospholipase A2 in mechanisms related to stimulated arachidonic acid release was investigated. We could parallel the overexpression of phospholipase A2 with an increase in phorbol ester and fluoroaluminate-stimulated arachidonic acid release. Pertussis toxin inhibited this stimulation. These results suggest that the 14 kDa type II phospholipase A2 might contribute to stimulation of arachidonic acid release, and therefore to eicosanoid production. © 1991.

引用

页码：1298 / 1305

页数：8

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