PREPARATION OF 5-(AMINOMETHYL)FLUORESCEIN AND 6-(AMINOMETHYL)FLUORESCEIN

被引：27

作者：

MATTINGLY, PG

机构：

[1] Abbott Laboratories, Diagnostics Division, Abbott Park, Illinois 60064, One Abbott Park Road

来源：

BIOCONJUGATE CHEMISTRY | 1992年 / 3卷 / 05期

关键词：

D O I：

10.1021/bc00017a013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

5(6)-Carboxyfluorescein is protected as the diacetate then reduced to 5(6)-(hydroxymethyl)fluorescein diacetate. The separated isomers are subjected to a Mitsunobu reaction with dibenzyl imidodicarbonate, yielding diprotected 5-and 6-(aminomethyl)fluorescein diacetate. Methanolysis of the acetates followed by deprotection with HBr/acetic acid gives 5- and 6-(aminomethyl)fluorescein hydrobromide.

引用

页码：430 / 431

页数：2

共 13 条

[1]

Brown, 1972, BORANES ORGANIC CHEM

[2] CARBOXYFLUORESCEIN FLUOROCHROMASIA ASSAYS .1. NON-RADIOACTIVELY LABELED CELL-MEDIATED LYMPHOLYSIS [J].