MEMBRANE PERMEABLE FLUOROGENIC RHODAMINE SUBSTRATES FOR SELECTIVE DETERMINATION OF CATHEPSIN-L

被引：38

作者：

ASSFALGMACHLEIDT, I

ROTHE, G

KLINGEL, S

BANATI, R

MANGEL, WF

VALET, G

MACHLEIDT, W

机构：

[1] MAX PLANCK INST BIOCHEM,ARBEITSGRP ZELLBIOCHEM,W-8033 MARTINSRIED,GERMANY

[2] MAX PLANCK INST PSYCHIAT,NEUROMORPHOL ABT,W-8033 MARTINSRIED,GERMANY

[3] BROOKHAVEN NATL LAB,DEPT BIOL,UPTON,NY 11973

来源：

BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1992年 / 373卷 / 07期

关键词：

D O I：

10.1515/bchm3.1992.373.2.433

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dipeptidyl rhodamine diamide substrates (Z-Phe-Arg)2-R110 and (Z-Arg-Arg)2-R110 are 820- and 360-fold more selective for cathepsin L than for cathepsin B allowing a sensitive determination of cathepsin L activity in the presence of high activity of cathepsin B. The results obtained with cell lysates suggest that the cysteine proteinase activity of vital macrophages detected by flow cytometry with these substrates is mainly due to cathepsin L.

引用

页码：433 / 440

页数：8

共 19 条

[1]

ASSFALGMACHLEIDT I, 1990, BIOL CHEM H-S, V371, P211

[2]

ASSFALGMACHLEIDT I, 1988, BIOL CHEM H-S, V369, P263

[3] RESPIRATORY BURST ACTIVITY IN BRAIN MACROPHAGES - A FLOW CYTOMETRIC STUDY ON CULTURED RAT MICROGLIA [J].