CHARACTERIZATION OF POTENTIAL REGULATORY ELEMENTS WITHIN THE RAT PREPROTACHYKININ-A PROMOTER

被引：30

作者：

MENDELSON, SC ^{[1
]}

QUINN, JP ^{[1
]}

机构：

[1] ROYAL EDINBURGH & ASSOCIATED HOSP,MRC,BRAIN METAB UNIT,EDINBURGH EH10 5HF,MIDLOTHIAN,SCOTLAND

来源：

NEUROSCIENCE LETTERS | 1995年 / 184卷 / 02期

关键词：

TRANSCRIPTION; TACHYKININ; AP-1; BHLH; AP-2; SPL; SUBSTANCE P;

D O I：

10.1016/0304-3940(94)11186-M

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The rat preprotachykinin-A (rPPT-A) gene encodes the precursor of several tachykinin neuropeptides including substance P. Previous studies have demonstrated the presence of multiple DNA sequences important for directing expression of the rPPT-A gene in dorsal root ganglion neurons within a region of the promoter spanning nucleotides -865 and -47. In order to identify potential cis acting elements, we have carried out DNase 1 footprinting analysis using a series of constructs containing fragments from this region of the promoter. This study has defined three potential AP-1 complex interactions, two E box binding protein interactions and two dG rich elements, which are potentially bound by complexes related to AP-2 or Sp1 in this region of the promoter.

引用

页码：125 / 128

页数：4

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