SOLUBILITY OF HYDROPHOBIC SURFACTANT PROTEINS IN ORGANIC-SOLVENT WATER MIXTURES - STRUCTURAL STUDIES ON SP-B AND SP-C IN AQUEOUS-ORGANIC SOLVENTS AND LIPIDS

The solubility of hydrophobic pulmonary surfactant proteins in different organic solvents and organic solvent/water combinations has been analyzed. Three organic solvents have been selected: methanol (MetOH), acetonitrile (ACN) and trifluorethanol (TFE). Porcine SP-B showed very similar calculated secondary structure when dissolved in methanol, 60% ACN or 70% TFE and reconstituted in lysophosphatidylcholine (LPC) micelles or dipalmitoylphosphatidylcholine (DPPC) vesicles, as deduced from circular dichroism studies. SP-B was calculated to posses around 45% of alpha-helix in all these systems. The fluorescence emission spectrum of SP-B has been also characterized in aqueous solvents and lipids. It always showed a splitting of the tryptophan contribution into two components with different emission maxima. SP-C had a very different structure in 80% ACN or 70% TFE. While alpha-helix was the main secondary structure of SP-C in ACN/water mixtures - around 50% -, it had almost exclusively beta-structure when dissolved in 70% TFE. The CD spectrum of SP-C in TFE showed dependence on the protein concentration, suggesting that protein-protein interactions could be important in this beta-conformation. SP-C reconstituted in LPC micelles or DPPC vesicles had a CD spectrum qualitatively similar to that one in aqueous ACN, with a dominant alpha-helical structure. The alpha-helical content of SP-C in micelles of LPC and vesicles of DPPC, 60 and 70%, respectively, was calculated to be higher than the alpha-helical content of the protein dissolved in any aqueous organic solvent.