CONTRACTILE PROPERTIES OF THE DEVELOPING DIAPHRAGM CORRELATE WITH MYOSIN HEAVY-CHAIN PHENOTYPE

被引:69
作者
JOHNSON, BD
WILSON, LE
ZHAN, WZ
WATCHKO, JF
DAOOD, MJ
SIECK, GC
机构
[1] MAYO CLIN & MAYO FDN,DEPT PHYSIOL & BIOPHYS,ROCHESTER,MN 55905
[2] UNIV PITTSBURGH,MAGEE WOMENS HOSP,SCH MED,DEPT PEDIAT,PITTSBURGH,PA 15213
关键词
MATURATION; MUSCLE PROTEIN COMPOSITION; VELOCITY OF SHORTENING; SPECIFIC FORCE;
D O I
10.1152/jappl.1994.77.1.481
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The objective of this study was to determine the relationship between developmental transitions in myosin heavy chain (MHC) composition and changes in maximum unloaded shortening velocity (V-o) and maximum specific force (P-o) of the rat diaphragm muscle. The diaphragm was excised at postnatal days 0, 3, 7, 14, 21, and 28 and in adults. MHC isoform expression was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and laser densitometry. In muscle fiber bundles, V-o was determined at 15 degrees C by use of the ''slack'' test. Isometric P-o was determined at 15 and 26 degrees C. Simple and step wise regressions were used to evaluate the correlations between V-o, P-o, and MHC phenotype transitions and the various developmental ages. The progressive increases in V-o and P-o with age were found to be inversely correlated to MHC-neonatal isoform expression (r(2) = -0.84 and -0.63, respectively) and positively correlated to MHC-2X (r(2) = 0.78 and 0.57) and MHC-2B (r(2) = 0.51 and 0.40) isoform expression (P < 0.001). Changes in MHC-neonatal isoform expression contributed to most of the developmental variance in V-o and P-o, with changes in MHC-2X and MHC-2B expression also contributing significant increments to total variance. The postnatal increase in V-o most likely relates to differences in the actomyosin adenosinetriphosphatase activity between neonatal and adult fast MHC phenotypes. The increase in P-o may reflect inherent differences in myofibrillar density, cross-bridge cycling kinetics, and/or the force produced per cross bridge among fibers composed of the different MHC isoforms.
引用
收藏
页码:481 / 487
页数:7
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