BINDING OF MISMATCHED MICROSATELLITE DNA-SEQUENCES BY THE HUMAN MSH2 PROTEIN

被引：161

作者：

FISHEL, R ^{[1
]}

EWEL, A ^{[1
]}

LEE, S ^{[1
]}

LESCOE, MK ^{[1
]}

GRIFFITH, J ^{[1
]}

机构：

[1] UNIV N CAROLINA,LINEBERGER CANC RES CTR,DEPT MICROBIOL,CHAPEL HILL,NC 27599

来源：

SCIENCE | 1994年 / 266卷 / 5189期

关键词：

D O I：

10.1126/science.7973733

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Alteration of the human mismatch repair gene hMSH2 has been linked to the microsatellite DNA instability found in hereditary nonpolyposis colon cancer and several sporadic cancers. This microsatellite DNA instability is thought to arise from defective repair of DNA replication errors that create insertion-deletion loop-type (IDL) mismatched nucleotides. Here, it is shown that purified hMSH2 protein efficiently and specifically binds DNA containing IDL mismatches of up to 14 nucleotides. These results support a direct role for hMSH2 in mutation avoidance and microsatellite stability in human cells.

引用

页码：1403 / 1405

页数：3

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