MODIFIED RADIOISOTOPIC ASSAY FOR MEASURING GLUTAMINE-SYNTHETASE ACTIVITY IN TISSUE-EXTRACTS

被引：119

作者：

PISHAK, MR ^{[1
]}

PHILLIPS, AT ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT BIOCHEM & BIOPHYS,ALTHOUSE LAB,UNIVERSITY PK,PA 16802

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90793-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A radioisotope assay for the measurement of glutamine synthetase activity has been developed in which tandemly arranged ion-exchange columns of Dowex 1-acetate and Amberlite CG-50 (H+) are used to separate the product, [14C]glutamine, from unreacted [U-14C]glutamate and other labeled compounds, particularly γ-aminobutyrate, that are formed by competing reactions. The technique is sensitive, reproducible, and suitable for multiple determinations. The assay has been used successfully to measure glutamine synthetase activity in neural and nonneural tissues which contain appreciable amounts of glutamate decarboxylase activity. © 1979.

引用

页码：82 / 88

页数：7

共 16 条

[1] L-GLUTAMIC ACID DECARBOXYLASE IN NON-NEURAL TISSUES OF MOUSE
DRUMMOND, RJ
PHILLIPS, AT
[J]. JOURNAL OF NEUROCHEMISTRY, 1974, 23 (06) : 1207 - 1213
[2] TRITOSOL - NEW SCINTILLATION COCKTAIL BASED ON TRITON X-100
FRICKE, U
[J]. ANALYTICAL BIOCHEMISTRY, 1975, 63 (02) : 555 - 558
[3] COMPARTMENTATION AND LABELING OF AMINO-ACIDS IN DEVELOPING RAT-BRAIN AFTER INJECTION OF [U-C-14]RIBOSE
GAITONDE, MK
KELLY, R
[J]. JOURNAL OF NEUROCHEMISTRY, 1972, 19 (02) : 377 - &
[4] Ginsburg A, 1973, ENZYMES GLUTAMINE ME, P9
[5] REGULATION GLUTAMINE SYNTHETASE .11. NATURE AND IMPLICATIONS OF A LAG PHASE IN ESCHERICHIA COLI GLUTAMINE SYNTHETASE REACTION
KINGDON, HS
HUBBARD, JS
STADTMAN, ER
[J]. BIOCHEMISTRY, 1968, 7 (06) : 2136 - &
[6] KULKA RG, 1973, J BIOL CHEM, V248, P6738
[7] LOWRY OH, 1951, J BIOL CHEM, V193, P265
[8] Meister A., 1970, METHOD ENZYMOL, V17, P900, DOI 10.1016/0076-6879(71)17304-1
[9] METABOLISM OF GAMMA-AMINOBUTYRIC ACID (GABA) IN LOBSTER NERVOUS SYSTEM - GLUTAMIC DECARBOXYLASE
MOLINOFF, PB
KRAVITZ, EA
[J]. JOURNAL OF NEUROCHEMISTRY, 1968, 15 (05) : 391 - &
[10] MORIN AM, 1977, J NEUROCHEM, V31, P371

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