FACTORS THAT INFLUENCE THE DEOXYRIBOSE OXIDATION ASSAY FOR FENTON REACTION-PRODUCTS

被引：51

作者：

WINTERBOURN, CC

机构：

[1] Department of Pathology, Christchurch School of Medicine, Christchurch Hospital, Christchurch

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1991年 / 11卷 / 04期

基金：

英国医学研究理事会;

关键词：

HYDROXYL RADICAL; FENTON SYSTEMS; IRON; DEOXYRIBOSE;

D O I：

10.1016/0891-5849(91)90151-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism of oxidation of deoxyribose to thiobarbituric acid-reactive products by Fenton systems consisting of H2O2 and either Fe2+ or Fe2+ (EDTA) has been studied. With Fe2+ (EDTA), dependences of product yield on reactant concentrations are consistent with a reaction involving OH.. With Fe2+ in 5-50 mM phosphate buffer, yields of oxidation products were much higher and increased with increasing deoxyribose concentration up to 30 mM. The product yield varied with H2O2 and Fe2+ concentrations in a way to suggest competition between deoxyribose and both reactants. Deoxyribose oxidation by Fe2+ and H2O2 was enhanced 1.5-fold by adding superoxide dismutase, even though superoxide generated by xanthine oxidase increased deoxyribose oxidation. These results are not as expected for a reaction involving free OH. or site localized OH. produced on the deoxyribose. They can be accommodated by a mechanism of deoxyribose oxidation involving an iron(IV) species formed from H2O2 and Fe2+, but the overall conclusion is that the system is too complex for definitive identification of the Fenton oxidant.

引用

页码：353 / 360

页数：8

共 32 条

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