ANTI-GLYCOSYL ANTIBODIES - ANTIBODIES DIRECTED AGAINST THE CARBOHYDRATE MOIETIES OF A GLYCOPROTEIN

被引：11

作者：

PAZUR, JH ^{[1
]}

FORRY, KR ^{[1
]}

TOMINAGA, Y ^{[1
]}

BALL, EM ^{[1
]}

机构：

[1] PENN STATE UNIV, PAUL M ALTHOUSE LAB, UNIVERSITY PK, PA 16802 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1981年 / 100卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(81)80113-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucoamylase [GA, EC 3.2.1.3] is an unusual type of glycoprotein containing 20 single mannose units and 24 oligosaccharide chains of mannose, glucose and galactose which are attached O-glycosidically to Ser and Thr residues of the protein. The immunogenicity of GA resided in the oligosaccharide chains. Antibodies [rabbit] specific for the oligosaccharide moieties of GA were isolated by affinity chromatography on an adsorbent of Sepharose with GA ligands. Such antibodies should prove useful for studying the mechanism and the locale of glycosylation of a nascent protein during glycoprotein biosynthesis.

引用

页码：420 / 426

页数：7

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