DETECTION OF HIV-1 RNA IN HEPARINIZED PLASMA OF HIV-1 SEROPOSITIVE INDIVIDUALS

被引：18

作者：

IMAI, H ^{[1
]}

YAMADA, O ^{[1
]}

MORITA, S ^{[1
]}

SUEHIRO, S ^{[1
]}

KURIMURA, T ^{[1
]}

机构：

[1] NIPPON ZOKI PHARMACEUT CO LTD,INST BIOACT SCI,DIV BIOCHEM,DEPT CELL BIOL & MICROBIOL,OSAKA,JAPAN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 36卷 / 02期

关键词：

HEPARIN; HEPARINASE; REVERSE TRANSCRIPTION; PCR; GLASS POWDER;

D O I：

10.1016/0166-0934(92)90149-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The interference of reverse transcription by heparin was removed by heparinase. When the HIV-1 RNA in the presence of heparin was detected by a combination of reverse transcription and the polymerase chain reaction (PCR), heparinase treatment followed by removal of Ca2+ before the reverse transcription step permitted the efficient detection of HIV-1 RNA. Prior treatment with heparinase revealed HIV-1 RNA in 68% (13/19) of heparinized plasma samples from HIV-1 carriers, whereas only 26% (5/19) of the same specimens were positive without the heparinase step. Heparinase removed the inhibition of reverse transcription by heparin and is highly recommended when detecting low levels of viral RNA in heparinized plasma.

引用

页码：181 / 184

页数：4

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