ELISAS FOR PARASITOLOGISTS - OR LIES, DAMNED LIES AND ELISAS

被引：40

作者：

VENKATESAN, P

WAKELIN, D

机构：

[1] Pradhib Venkateson and Derek Wokelin are, the Department of Life Science, University of Nottingham, Nottingham

来源：

PARASITOLOGY TODAY | 1993年 / 9卷 / 06期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0169-4758(93)90020-G

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The introduction in 1971, by Engvoll and Perlmann 1, of enzyme labels in immunoassays represented a significant technical advance. Their enzyme-linked immunosorbent assay (ELISA) proved to be as sensitive as radioimmunoossoys but safer to use. Since then, ELISAs have been widely used in the assay o f anti-bodies and antigens. In this article, Pradhib Venkateson and Derek Wokelin focus on some o f the problems associated with ELISAs when applied to parasitic infections, which can modify antibody responses, by immunosuppression or polyclonal activation. The article also considers optimization, which is an essential step in the establishment o f on ELISA. © 1993.

引用

页码：228 / 232

页数：5

共 37 条

[1] ASSESSMENT OF SUBSTRATES FOR HORSERADISH-PEROXIDASE IN ENZYME-IMMUNOASSAY [J].

ALKAISSI, E ;

MOSTRATOS, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 58 (1-2) :127-132

[2] ANALYSIS OF THE OPTIMAL CONDITIONS FOR THE ADSORPTION OF TYPE-III PNEUMOCOCCAL POLYSACCHARIDE TO PLASTIC FOR USE IN SOLID-PHASE ELISA [J].

ELKINS, KL ;

STASHAK, PW ;

BAKER, PJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 130 (01) :123-131

[3] ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) QUANTITATIVE ASSAY OF IMMUNOGLOBULIN-G [J].

ENGVALL, E ;

PERLMANN, P .

IMMUNOCHEMISTRY, 1971, 8 (09) :871-&

[4] COATING OF PROTEINS TO POLYSTYRENE ELISA PLATES IN THE PRESENCE OF DETERGENTS [J].

GARDAS, A ;

LEWARTOWSKA, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 106 (02) :251-255

[5] EFFICIENT DIAGNOSIS OF GIARDIASIS AMONG NURSERY AND PRIMARY-SCHOOL CHILDREN IN SANTIAGO, CHILE BY CAPTURE ELISA FOR THE DETECTION OF FECAL GIARDIA ANTIGENS [J].

GOLDIN, AJ ;

APT, W ;

AGUILERA, X ;

ZULANTAY, I ;

WARHURST, DC ;

MILES, MA .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 42 (06) :538-545

[6]

GREENWOOD BM, 1974, LANCET, V1, P435

[7] IMMUNODIAGNOSIS OF GIARDIASIS BY ELISA AND STUDIES ON CROSS-REACTIVITY BETWEEN THE ANTI-GIARDIA-LAMBLIA ANTIBODIES AND SOME HETEROLOGOUS PARASITIC ANTIGENS AND FRACTIONS [J].

HARALABIDIS, ST .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1984, 78 (03) :295-300

[8] TITRATION OF ANTIBODIES [J].

KEMENY, DM .

JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 150 (1-2) :57-76

[9] PRINCIPLES, PROBLEMS, AND STRATEGIES IN THE USE OF ANTIGENIC MIXTURES FOR THE ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].

KENNY, GE ;

DUNSMOOR, CL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 17 (04) :655-665

[10]

KILSHAW PJ, 1986, CLIN EXP IMMUNOL, V66, P481

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