A PARAFFIN-CELLOIDIN EMBEDDING METHOD FOR STUDYING SOFT-HARD TISSUE INTERFACES

被引:5
作者
LINDER, JE
LONGHURST, P
JOHNSON, NW
机构
[1] UNIV LONDON LONDON HOSP, COLL MED, DEPT ORAL PATHOL, LONDON E1 1BB, ENGLAND
[2] GUYS HOSP, DEPT ORTHODONT & DENT CHILDREN, LONDON SE1 9RT, ENGLAND
关键词
D O I
10.1016/0003-9969(81)90193-X
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Formaldehyde-fixed [human] tooth-gingiva specimens were embedded whole in low-viscosity nitrocellulose (celloidin) to maintain the soft-hard tissue relationships. The soft tissue-bearing portion was sliced off, demineralized in EDTA and re-infiltrated in paraffin wax. Sections were mounted using a special benzyl alcohol acetone-containing fluid to achieve complete flattening and successfully stained using a variety of stains.
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页码:753 / 755
页数:3
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